Multiple ligation-Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2Article Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification Analysis Bacterial colorimetric detection Colorimetric sensing conditions coronavirus detection system diagnosing disease Diseases effective Genome isothermal isothermal amplification limit of detection Multiple PK-probe point-of-care polymerase probe Pyrophosphate recombinase polymerase amplification Recombinase polymerase amplification (RPA) RNA robust SARS-CoV-2 SARS-CoV-2. selective sensitivity [DOI] 10.1016/j.talanta.2022.123835 PMC 바로가기
Evaluation of indirect sequence-specific magneto-extraction-aided LAMP for fluorescence and electrochemical SARS-CoV-2 nucleic acid detectionArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification approach circumvent clinically Disadvantages Electrochemical LAMP Host in silico inhibitor investigated isothermal isothermal amplification Magnetic sequence-specific target enrichment molecular NAAT nucleic acid Nucleic acid detection PCR polymerase probe qRT-PCR Quantitative Quantitative real-time LAMP real-time reverse transcriptase restrict SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2 variant SARS-CoV-2. sensitivity sequence serum turn [DOI] 10.1016/j.talanta.2022.123809 PMC 바로가기
Ultrasensitive monitoring of SARS-CoV-2-specific antibody responses based on a digital approach reveals one week of IgG seroconversionArticle Published on 2022-12-012022-11-15 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] addition antibodies Antibody Response approach AUC collected COVID-19 COVID-19 patient diagnostic diagnostics Donor Extreme early-stage seroconversion faster flow cytometry healthy IgG IgG and IgM IgG/IgM antibodies IgM immunoassay immunoassays immunological inactivated nucleic acid nucleic acids other diseases pathogen-specific Patient peptide platform probe Protein RBD reveal Rhesus monkeys SARS-CoV-2 S2 sensitivity Seroconversion seroconversion time Simoa tested Ultrasensitive digital detection. vaccination virus wild-type [DOI] 10.1016/j.bios.2022.114710 PMC 바로가기
Highly sensitive and rapid detection of SARS-CoV-2 via a portable CRISPR-Cas13a-based lateral flow assayArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome addition amplification approach assays clinical sample comparable component Concentration coronavirus disease Coronavirus-2 COVID-19 CRISPR Diagnostic method Effectiveness ENhance examined identify immunochromatographic IMPROVE individual isothermal lateral flow lateral flow assay lateral flow strip limit of detection molecular polymerase chain primer probe Protein provide Quality control reliability Result reverse transcription RNA RT-PCR SARS-CoV-2 screened sensitive detection. sensitivity significantly Spread tested virus detection [DOI] 10.1002/jmv.28096 PMC 바로가기
Sudden unexpected death in a 17-year-old boy due to unacknowledged adamantinoma-like Ewing sarcomaArticle Published on 2022-12-012022-11-15 Journal: Forensic Science, Medicine, and Pathology [Category] COVID19(2023년), SARS, 바이오마커, 진단, 치료기술, [키워드] Abnormalities Adamantinoma-like Ewing sarcoma addition airway Airway occlusion Analysis analysis anatomical Autopsy Blood Brain break cardiac cause CD99 Cell characterized Chest radiography Complete death diagnosed diagnostic diagnostics died discharged disease Dyspnea EWSR1 excluded expression Forensic Forensic autopsy forensic pathology Germany histopathological hospital immunohistochemistry Invasion Leica Local lung macroscopic male mechanism Microscopy Mild molecular Nasopharyngeal swab neoplasm nucleoli organ oxygen saturation pathology probe SARS-CoV-2 Soft tissue subject sudden Sudden unexpected death. tested Thyroid was performed [DOI] 10.1007/s12024-022-00525-x PMC 바로가기
Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2Article Published on 2022-11-152022-10-06 Journal: Sens Actuators B Chem [Category] 진단, [키워드] acute respiratory syndrome amplification approach caused coronavirus COVID-19 COVID-19 pandemic cross-contamination Detection limit diagnostics Dumbbell-type triplex molecular switch Epidemics Frame gold standard High-fidelity independent Logic-gate molecular Molecular assay N gene nucleic acid amplification ORF1ab probe recognizing Region RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 RNA. spacer [DOI] 10.1016/j.snb.2022.132579 PMC 바로가기 [Article Type] Article
Molecular interactions and inhibition of the SARS-CoV-2 main protease by a thiadiazolidinone derivativeArticle Published on 2022-11-012022-11-15 Journal: Proteins [Category] COVID19(2023년), SARS, 치료제, [키워드] comparable conformational docking enzyme inhibitor Interaction involved Local main protease molecular molecular dynamics Molecular dynamics simulation molecular interaction Pro probe protease protocol SARS-CoV-2 the SARS-CoV-2 thiadiazolidinone. viral life cycle [DOI] 10.1002/prot.26385 PMC 바로가기
Bioinformatics pipeline unveils genetic variability to synthetic vaccine design for Indian SARS-CoV-2 genomesArticle Published on 2022-11-012022-11-15 Journal: International immunopharmacology [Category] SARS, 치료기술, 치료제, [키워드] aligned amino acid change antigenic antigenic epitopes B-cell epitope bioinformatics bioinformatics pipeline can be used clade clades conserved conserved regions criteria epitope Evolution genetic variability genomic HLA allele identify immunogenic MAFFT MHC-I MHC-II Multiple sequence alignment Mutation Nextstrain Non-synonymous Non-Synonymous Signature SNP performed Phylogenetic analysis primer probe Protein Region regions reported resulting SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 pandemic selected SNP SNPs T-cell T-cell epitope T-cell epitopes T-cell epitopes. unique Vaccine Vaccine design virus virus detection virus genome [DOI] 10.1016/j.intimp.2022.109224 PMC 바로가기
Electrochemiluminescence resonance energy transfer biosensing platform between g-C3N4 nanosheet and Ru-SiO2@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp geneArticle Published on 2022-11-012022-11-15 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] amplification binding affinity Clinical diagnosis coronavirus Detection sensitivity disease DNA ECL Electrochemiluminescence energy transfer limit of detection multifunctional platform probe RdRp gene RdRp gene biosensor g-C(3)N(4) nanosheets. RET SARS-CoV-2 SARS-CoV-2 RdRp SARS-CoV-2 virus ssDNA syndrome target gene Treatment unique was used [DOI] 10.1016/j.bios.2022.114580 PMC 바로가기
Development of multiplex S-gene-targeted RT-PCR for rapid identification of SARS-CoV-2 variants by extended S-gene target failureArticle Published on 2022-11-012022-11-15 Journal: Clinica chimica acta; international journal of cli [Category] SARS, 변종, 치료기술, 치료제, [키워드] binding caused codon concordance rate Delta development Epidemics genomic sequencing Internal control Japan multiplex Multiplex RT-PCR Mutation N-gene omicron probe Rapid Rapid screening Region RT-PCR S-gene S-gene mutations S-gene target failure SARS-CoV-2 SARS-CoV-2 variant screening method Sequencing time-consuming Variant of concern. VoC VOCs [DOI] 10.1016/j.cca.2022.08.031 PMC 바로가기